Smart light-sheet with metabolic imaging for extremely delicate samples. The aim of this project is to develop advanced imaging technologies to allow long term visualisation and recording of individua

Description

Smart light-sheet with metabolic imaging for extremely delicate samples. The aim of this project is to develop advanced imaging technologies to allow long term visualisation and recording of individual organelles and cells within complex multicellular structures such as embryos, developing tissues, organoids and iblastoids. Imaging technologies have driven major advances in the life sciences but photo-toxicity has limited advances in sensitive multicellular systems. The technology we will implement reduces phototoxicity by using 2-photon microscopy and adaptive opticsn and an Airy beam scanning system enhances speed and resolution of imaging. This capability will lead to new discoveries in how organelles and cells interact to create functional multicellular systems. . Scheme: Linkage Infrastructure, Equipment and Facilities. Field: 3101 - Biochemistry and Cell Biology. Lead: Prof John Carroll